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KMID : 0624620140470060324
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BMB Reports
2014 Volume.47 No. 6 p.324 ~ p.329
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The GABAB receptor associates with regulators of G-protein signaling 4 protein in the mouse prefrontal cortex and hypothalamus
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Kim Gyeong-Wha
Jung Soon-Woong
Son Hyeon-Wi
Kim Su-Jeong
Choi Jung-Il
Lee Dong-Hoon
Roh Gu-Seob
Kang Sang-Soo
Cho Gyeong-Jae
Choi Wan-Sung
Kim Hyun-Joon
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Abstract
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Regulators of G-protein signaling (RGS) proteins regulate certain G-protein-coupled receptor (GPCR)-mediated signaling pathways. The GABAB receptor (GABABR) is a GPCR that plays a role in the stress response. Previous studies indicate that acute immobilization stress (AIS) decreases RGS4 in the prefrontal cortex (PFC) and hypothalamus (HY) and suggest the possibility of a signal complex composed of RGS4 and GABABR. Therefore, in the present study, we tested whether RGS4 associates with GABABR in these brain regions. We found the co-localization of RGS4 and GABABR subtypes in the PFC and HY using double immunohistochemistry and confirmed a direct association between GABAB2R and RGS4 proteins using co-immunoprecipitation. Furthermore, we found that AIS decreased the amount of RGS4 bound to GABAB2R and the number of double-positive cells. These results indicate that GABABR forms a signal complex with RGS4 and suggests that RGS4 is a regulator of GABABR.
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KEYWORD
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GABAB receptor, Hypothalamus, Prefrontal cortex, RGS4, Stress response
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